Journal: Biomedicines

Article Title: Beneficial Actions of 4-Methylumbelliferone in Type 1 Diabetes by Promoting β Cell Renewal and Inhibiting Dedifferentiation.

doi: 10.3390/biomedicines12122790

Figure Lengend Snippet: Figure 5. Effect of 4-MU on islet injury induced by STZ in vitro. (A) Representative images of AO/PI staining of primary islet, green fluorescence indicates living cells and red fluorescence indicates dead cells. Scale bars, 50 µm. (B) Representative images of scanning electron microscope of islets in different groups. Scale bars, 50 µm, 10 µm. (C) GSIS experiments in different groups in vitro. (D–F) Detection of mRNA level of β cells markers. Statistics were determined by two-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01 vs. the STZ.

Article Snippet: Slides were finally incubated with DAPI (1 μg/mL, Sigma-Aldrich) for 20 min at room temperature, and then mounted for imaging using NIKON Eclipse C1 confocal fluorescence microscope (Nikon, AX/AX R with NSPARC, Tokyo, Japan) fitted with DAPI (350 nm), FITC (488 nm) and TRITC (594 nm) filters.

Techniques: In Vitro, Staining, Fluorescence, Microscopy